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anti h3k4me1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti h3k4me1
    Anti H3k4me1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+h3k4me1/Mono-Methyl-Histone+H3+(Lys4)+XP+Rabbit+mAb/pm41832971-442-11-12
    Average 96 stars, based on 258 article reviews
    anti h3k4me1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: BRWD3 promotes KDM5 degradation to maintain H3K4 methylation levels
    Article Snippet: .. After blocking, cells were incubated with rabbit anti-H3K4me1 (Cell Signaling #5326, 1:1,000), anti-H3K4me2 (Cell Signaling #9725, 1:1,000), or anti-H3K4me3 (Active Motif # 39060, 1:1,000) antibodies overnight at 4 °C in blocking buffer. .. After washing with PBT, cells were incubated with goat Alexa fluorophore 568–conjugated anti-rabbit IgG secondary antibody (1:500, Life Technologies, # A11011) in blocking buffer for 1 h at room temperature.

    Article Title: BRWD3 promotes KDM5 degradation to maintain H3K4 methylation levels
    Article Snippet: .. After blocking, cells were incubated with either rabbit anti-H3K4me1 (Cell Signaling #5326, 1:1000), anti-H3K4me2 (Cell Signaling #9725, 1:1000) or anti-H3K4me3 (Active Motif # 39060, 1:1000) antibodies overnight at 4°C in blocking buffer. .. After washing with PBT, cells were incubated with goat Alexa fluorophore 568-conjugated anti-rabbit IgG secondary antibody (1:500, Life Technologies, # A11011) in blocking buffer for 1h at room temperature.

    Incubation:

    Article Title: BRWD3 promotes KDM5 degradation to maintain H3K4 methylation levels
    Article Snippet: .. After blocking, cells were incubated with rabbit anti-H3K4me1 (Cell Signaling #5326, 1:1,000), anti-H3K4me2 (Cell Signaling #9725, 1:1,000), or anti-H3K4me3 (Active Motif # 39060, 1:1,000) antibodies overnight at 4 °C in blocking buffer. .. After washing with PBT, cells were incubated with goat Alexa fluorophore 568–conjugated anti-rabbit IgG secondary antibody (1:500, Life Technologies, # A11011) in blocking buffer for 1 h at room temperature.

    Article Title: BRWD3 promotes KDM5 degradation to maintain H3K4 methylation levels
    Article Snippet: .. After blocking, cells were incubated with either rabbit anti-H3K4me1 (Cell Signaling #5326, 1:1000), anti-H3K4me2 (Cell Signaling #9725, 1:1000) or anti-H3K4me3 (Active Motif # 39060, 1:1000) antibodies overnight at 4°C in blocking buffer. .. After washing with PBT, cells were incubated with goat Alexa fluorophore 568-conjugated anti-rabbit IgG secondary antibody (1:500, Life Technologies, # A11011) in blocking buffer for 1h at room temperature.

    Chromatin Immunoprecipitation:

    Article Title: Prox1 dynamically regulates downstream targets and chromatin accessibility during venous to lymphatic endothelial cell transdifferentiation in the embryo
    Article Snippet: .. For ChIP, antibodies used were goat anti-Prox1 (R&D Systems, AF2727), rabbit anti-H3K4Me1 (Cell Signaling, #5326), rabbit anti-H3K27Me3 (Cell Signaling, #9733), rabbit anti-H3K27Ac (Cell Signaling, #8173), rabbit anti-H3K4Me3 (Cell Signaling, #9751). hLEC were harvested and processed for ChIP as previously described using a truChIPTM Low Cell Chromatin Shearing Kit with SDS Shearing Buffer (Covaris). ..

    Staining:

    Article Title: Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation
    Article Snippet: Fixation and permeabilization were performed using the True Nuclear fixation kit (BioLegend, Cat #424401) following the manufacturer recommendations. .. Cells were then stained with rabbit anti-H3K4me1 (Cell Signaling Technology, Catalog #5326), rabbit anti-H3K4me2 (Cell Signaling Technology, Catalog #9725), normal rabbit IgG (Isotype control, sc-2027) and Alexa Fluor 594 (Thermo Fisher Scientific # A-21207). .. Flow cytometry analysis was performed using BD LSR Fortessa X20 (Becton Dickinson, San Jose, CA, USA) using the 488 nm laser (60 mW) for FSC (380 V) and SSC (212 V), and 561 nm laser (50 mW) using a 610/20 nm bandpass filter and a PMT voltage of 390 for AF594 excitation/detection.

    Article Title: Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation.
    Article Snippet: Fixation and permeabilization were performed using the True Nuclear fixation kit (BioLegend, Cat #424401) following the manufacturer recommendations. .. Cells were then stained with rabbit anti-H3K4me1 (Cell Signaling Technology, Catalog #5326), rabbit antiH3K4me2 (Cell Signaling Technology, Catalog #9725), normal rabbit IgG (Isotype control, sc-2027) and Alexa Fluor 594 (Thermo Fisher Scientific # A21207). .. Flow cytometry analysis was performed using BD LSR Fortessa X20 (Becton Dickinson, San Jose, CA, USA) using the 488 nm laser (60 mW) for FSC (380 V) and SSC (212 V), and 561 nm laser (50 mW) using a 610/20 nm bandpass filter and a PMT voltage of 390 for AF594 excitation/detection.

    Control:

    Article Title: Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation
    Article Snippet: Fixation and permeabilization were performed using the True Nuclear fixation kit (BioLegend, Cat #424401) following the manufacturer recommendations. .. Cells were then stained with rabbit anti-H3K4me1 (Cell Signaling Technology, Catalog #5326), rabbit anti-H3K4me2 (Cell Signaling Technology, Catalog #9725), normal rabbit IgG (Isotype control, sc-2027) and Alexa Fluor 594 (Thermo Fisher Scientific # A-21207). .. Flow cytometry analysis was performed using BD LSR Fortessa X20 (Becton Dickinson, San Jose, CA, USA) using the 488 nm laser (60 mW) for FSC (380 V) and SSC (212 V), and 561 nm laser (50 mW) using a 610/20 nm bandpass filter and a PMT voltage of 390 for AF594 excitation/detection.

    Article Title: Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation.
    Article Snippet: Fixation and permeabilization were performed using the True Nuclear fixation kit (BioLegend, Cat #424401) following the manufacturer recommendations. .. Cells were then stained with rabbit anti-H3K4me1 (Cell Signaling Technology, Catalog #5326), rabbit antiH3K4me2 (Cell Signaling Technology, Catalog #9725), normal rabbit IgG (Isotype control, sc-2027) and Alexa Fluor 594 (Thermo Fisher Scientific # A21207). .. Flow cytometry analysis was performed using BD LSR Fortessa X20 (Becton Dickinson, San Jose, CA, USA) using the 488 nm laser (60 mW) for FSC (380 V) and SSC (212 V), and 561 nm laser (50 mW) using a 610/20 nm bandpass filter and a PMT voltage of 390 for AF594 excitation/detection.

    Western Blot:

    Article Title: Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin
    Article Snippet: .. Mouse anti-RCOR1 (NeuroMab, 75-039, WB 1:1000); Mouse anti-RCOR1 (BD Biosciences, #612146, IF: 1:100), Rabbit anti-RCOR1 (Abcam, ab183711, IP: 2 μg) rabbit anti-LSD1/KDM1 (Abcam, ab17721, WB: 1:1000, IF: 1:500, IP: 2 μg); rabbit anti-HDAC1 (Abcam, ab7028, WB: 1:5000, IF: 1:1000, IP: 2 μg); mouse anti-HDAC2 (Abcam, ab51832, WB: 1:1000, IF: 1:500); rabbit anti-EZH2 (Cell Signaling Technology #5246, WB: 1:1000), rabbit anti-HP1α (Cell Signaling Technology, #2616, WB 1:1000), rabbit anti-RPB1 NTD (Cell Signaling Technology, #14958, WB: 1:1000, IF: 1:500, IP: 2 μg), rabbit anti-phospho-RPB1 CTD (Ser2) (Cell Signaling Technology, #13499 WB: 1:1000, IF: 1:500), rabbit anti-phospho-RPB1 CTD (Ser5) (Cell Signaling Technology, #13523, WB: 1:1000, IF: 1:500), rabbit anti phosphor-RPB1 CTD (Ser7) (Cell Signaling Technology, #13780, WB: 1:1000, IF: 1:500), rabbit anti-H3 (Novus Biologicals, NB500-171, WB: 1:1000), rabbit anti-H3K4me1 (Cell Signaling Technology, #5326, WB:1:1000), rabbit anti-H3K4me2 (Cell Signaling Technology, #9725, WB: 1:5000); rabbit anti-H3K4me3 (Cell Signaling Technology, #9751, WB: 1:1000, IF: 1:500), rabbit anti-H3K9me3 (Abcam, ab8898, IF: 1:1000); rabbit anti-H3K27me3 (Active Motif, #39055, WB:1:1000, IF: 1:250), rabbit anti-H3K9ac (Cell Signaling Technology, #9649, WB: 1:1000), rabbit anti-H3K18ac (Cell Signaling Technology, #13998, WB: 1:1000, IF: 1:500), mouse anti-GAPDH (Cell Signaling Technology, #5174, WB: 1:10000), rabbit anti-H3R2me2a (Epigentek, #A-3714: WB: 1:500), rabbit anti-HA tag (Cell Signaling Technology, #3724, WB:1:5000), rabbit anti-NCOR2 (Invitrogen PA1-843, WB: 1:1000), rabbit anti-MEK1/2 (Cell Signaling Technology, #8727, WB: 1:1000), mouse anti-β Tubulin (Sigma Aldrich #T5201, WB: 1:5000), rabbit anti EGFR (Cell Signaling Technology, #4267, IF: 1:500), rabbit anti Lamin B1 (Abcam, #ab65986, WB: 1:5000), rabbit anti-acetyl-Lysine (Active Motif, #91315, IP: 5 μg), rabbit anti-dimethyl-Lysine (Cell Signaling Technology, #14117, IP: 1:100). ..



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    ( a ) Volcano plot of ATAC-seq analysis. Shown are ATAC-seq all peaks increased (red) and decreased (blue) in BRD8-deficent Huh7 cells (FDR-adjusted p < 0.05). ( b ) The amounts of upregulated repeats in BRD8 knockout Huh7 cells. ( c ) Binding density heatmap of ATAC-seq, H3K27ac, H3K9me3, H4K20me3 and H3K27me3 ChIP-seq signals at ATAC-up_TE region in WT or BRD8-deficent Huh7 cells. ( d ) Binding density heatmap of H3K27ac ChIP-seq signals at BRD8-binding region , ATAC-up region or all H3K27ac peaks region in WT or BRD8-deficent Huh7 cells. ( e ) Binding density heatmap of H3K4me3 or <t>H3K4me1</t> ChIP-seq signals at BRD8-binding region or ATAC-up region in WT or BRD8-deficent Huh7 cells. ( f ) Binding density heatmap of SMARCA4 ChIP-seq signals at ATAC-up region in WT or BRD8-deficent Huh7 cells. ( g ) RT-qPCR analyses of TEs mRNA levels in Huh7 cells upon BRD8 depletion with or without AU-15330 treatment for 72 h. Results is shown as mean ± SD (n = 3). ( h ) Flow cytometry histograms of dsRNA staining of WT or BRD8 KO Huh7 cells with or without AU-15330 treatment for 72 h. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired Student’s t -test.
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    ( a ) Volcano plot of ATAC-seq analysis. Shown are ATAC-seq all peaks increased (red) and decreased (blue) in BRD8-deficent Huh7 cells (FDR-adjusted p < 0.05). ( b ) The amounts of upregulated repeats in BRD8 knockout Huh7 cells. ( c ) Binding density heatmap of ATAC-seq, H3K27ac, H3K9me3, H4K20me3 and H3K27me3 ChIP-seq signals at ATAC-up_TE region in WT or BRD8-deficent Huh7 cells. ( d ) Binding density heatmap of H3K27ac ChIP-seq signals at BRD8-binding region , ATAC-up region or all H3K27ac peaks region in WT or BRD8-deficent Huh7 cells. ( e ) Binding density heatmap of H3K4me3 or <t>H3K4me1</t> ChIP-seq signals at BRD8-binding region or ATAC-up region in WT or BRD8-deficent Huh7 cells. ( f ) Binding density heatmap of SMARCA4 ChIP-seq signals at ATAC-up region in WT or BRD8-deficent Huh7 cells. ( g ) RT-qPCR analyses of TEs mRNA levels in Huh7 cells upon BRD8 depletion with or without AU-15330 treatment for 72 h. Results is shown as mean ± SD (n = 3). ( h ) Flow cytometry histograms of dsRNA staining of WT or BRD8 KO Huh7 cells with or without AU-15330 treatment for 72 h. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired Student’s t -test.
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    ( a ) Volcano plot of ATAC-seq analysis. Shown are ATAC-seq all peaks increased (red) and decreased (blue) in BRD8-deficent Huh7 cells (FDR-adjusted p < 0.05). ( b ) The amounts of upregulated repeats in BRD8 knockout Huh7 cells. ( c ) Binding density heatmap of ATAC-seq, H3K27ac, H3K9me3, H4K20me3 and H3K27me3 ChIP-seq signals at ATAC-up_TE region in WT or BRD8-deficent Huh7 cells. ( d ) Binding density heatmap of H3K27ac ChIP-seq signals at BRD8-binding region , ATAC-up region or all H3K27ac peaks region in WT or BRD8-deficent Huh7 cells. ( e ) Binding density heatmap of H3K4me3 or <t>H3K4me1</t> ChIP-seq signals at BRD8-binding region or ATAC-up region in WT or BRD8-deficent Huh7 cells. ( f ) Binding density heatmap of SMARCA4 ChIP-seq signals at ATAC-up region in WT or BRD8-deficent Huh7 cells. ( g ) RT-qPCR analyses of TEs mRNA levels in Huh7 cells upon BRD8 depletion with or without AU-15330 treatment for 72 h. Results is shown as mean ± SD (n = 3). ( h ) Flow cytometry histograms of dsRNA staining of WT or BRD8 KO Huh7 cells with or without AU-15330 treatment for 72 h. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired Student’s t -test.
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    Image Search Results


    ( a ) Volcano plot of ATAC-seq analysis. Shown are ATAC-seq all peaks increased (red) and decreased (blue) in BRD8-deficent Huh7 cells (FDR-adjusted p < 0.05). ( b ) The amounts of upregulated repeats in BRD8 knockout Huh7 cells. ( c ) Binding density heatmap of ATAC-seq, H3K27ac, H3K9me3, H4K20me3 and H3K27me3 ChIP-seq signals at ATAC-up_TE region in WT or BRD8-deficent Huh7 cells. ( d ) Binding density heatmap of H3K27ac ChIP-seq signals at BRD8-binding region , ATAC-up region or all H3K27ac peaks region in WT or BRD8-deficent Huh7 cells. ( e ) Binding density heatmap of H3K4me3 or H3K4me1 ChIP-seq signals at BRD8-binding region or ATAC-up region in WT or BRD8-deficent Huh7 cells. ( f ) Binding density heatmap of SMARCA4 ChIP-seq signals at ATAC-up region in WT or BRD8-deficent Huh7 cells. ( g ) RT-qPCR analyses of TEs mRNA levels in Huh7 cells upon BRD8 depletion with or without AU-15330 treatment for 72 h. Results is shown as mean ± SD (n = 3). ( h ) Flow cytometry histograms of dsRNA staining of WT or BRD8 KO Huh7 cells with or without AU-15330 treatment for 72 h. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired Student’s t -test.

    Journal: bioRxiv

    Article Title: BRD8 Disruption Unlocks Retrotransposon-Driven Tumor-Intrinsic Immunogenicity via Redistribution of Histone Acetylation in Liver Cancer

    doi: 10.64898/2026.01.21.700714

    Figure Lengend Snippet: ( a ) Volcano plot of ATAC-seq analysis. Shown are ATAC-seq all peaks increased (red) and decreased (blue) in BRD8-deficent Huh7 cells (FDR-adjusted p < 0.05). ( b ) The amounts of upregulated repeats in BRD8 knockout Huh7 cells. ( c ) Binding density heatmap of ATAC-seq, H3K27ac, H3K9me3, H4K20me3 and H3K27me3 ChIP-seq signals at ATAC-up_TE region in WT or BRD8-deficent Huh7 cells. ( d ) Binding density heatmap of H3K27ac ChIP-seq signals at BRD8-binding region , ATAC-up region or all H3K27ac peaks region in WT or BRD8-deficent Huh7 cells. ( e ) Binding density heatmap of H3K4me3 or H3K4me1 ChIP-seq signals at BRD8-binding region or ATAC-up region in WT or BRD8-deficent Huh7 cells. ( f ) Binding density heatmap of SMARCA4 ChIP-seq signals at ATAC-up region in WT or BRD8-deficent Huh7 cells. ( g ) RT-qPCR analyses of TEs mRNA levels in Huh7 cells upon BRD8 depletion with or without AU-15330 treatment for 72 h. Results is shown as mean ± SD (n = 3). ( h ) Flow cytometry histograms of dsRNA staining of WT or BRD8 KO Huh7 cells with or without AU-15330 treatment for 72 h. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired Student’s t -test.

    Article Snippet: After that, the supernatant was added Protein A/G beads pre-bound with 3-5 μg of specific antibodies against Flag-M2 (Sigma, A2220), SMARCA4 (Abcam, ab110641), H3K27ac (Abcam, ab4729), H3K4me3 (Abcam, ab8580), H2A.Z (Abcam, ab150402), H3K9me3 (Abcam, ab8898), H3K4me1 (CST, 5326), H3K27me3 (CST, 9733) and H4K20me3 (Abcam, ab9053) overnight.

    Techniques: Knock-Out, Binding Assay, ChIP-sequencing, Quantitative RT-PCR, Flow Cytometry, Staining